The key to AxoFuse® is an adaption of a well established method used to induce the fusion of cells in vitro. Low molecular weight polyethylene glycol (PEG) temporarily alters the biophysical properties of the plasma membranes of cells permitting PEG-treated cells, when in close approximation, to fuse.
PEG-fusion was adapted to the fusion of neurons through several years of research and development. The method has been fully optimized for use in vivo. The process now incorporates other steps to allow a high percentage of severed axons within a severed peripheral nerve to become fused.